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KMID : 0364819860240010001
Korean Journal of Microbiology
1986 Volume.24 No. 1 p.1 ~ p.5
Isolation and Identification of Tumor-inducing(Ti) Plasmids



Abstract
1
Tumor-inducing (Ti) plasmid which may be used in genetic engineering of higher plants, was isolated from Ag obacterium fume!aciens in Korea. Among the 7 strains of A. fume!aciens isolated from various regions in Korea, KU12, KU13, KU14, and KU49 strains were confirmed to contain plasmid. The isolated Ti plasmids were digested with 4 kinds of restriction enzymes, respectively. According to the result of the cleavage patterns, we confirmed that these plasmids in the KU12, KU13, KU14 and KU49 strains are different.
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